Background
Small intestinal submucosa (SIS) represents a novel bio-scaffolding material that
may be used to repair hollow-organ defects. However, it is unclear whether neurophysiologic
responses return to SIS-grafted areas in the gut. We evaluated the functional recovery
of a stomach defect grafted with the porcine-derived SIS.
Methods
Twelve rats had a full-thickness defect created in the stomach. SIS was secured to
the gastric wall. After 6 months, muscle strips were harvested from within the grafted
area to perform both a histologic and a functional study. Additional full-thickness
muscle strips were harvested from the posterior in the same stomach as controls. A
dose response curve was obtained with carbachol (CCH) or sodium nitroprusside (SNP).
Activation of intrinsic nerves was achieved by electrical field stimulation (EFS).
Results
The response to CCH and amplitude in EFS showed tonic contraction in both controls
and SIS strips in a concentration-dependent and frequency-dependent manner. The magnitude
after each stimulation was significantly lower in SIS strips compared with controls
(P < .01). However, the contraction ratio of EFS to ED50 of CCH was not significantly different between the groups. Additionally, SNP produced
relaxation in both strips in a concentration-dependent manner. Histologic findings
revealed that an insufficient amount of smooth-muscle cells existed in the muscularis
propria, whereas compensated growth was observed in the submucosa with nerve regeneration.
Conclusions
This study demonstrates that SIS provides a template for nerve migration to the graft
in the rodent stomach. Innervations showed a similar distribution to that observed
in the controls. The clinical implications of such findings warrant additional investigation.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to SurgeryAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Resorbable bioscaffold for esophageal repair in a dog model.J Pediatr Surg. 2000; 35: 1097-1103
- Use of a new elastin patch and glue for repair of a major duodenal injury.ASAIO J. 2000; 46: 409-414
- Small bowel tissue engineering using small intestinal submucosa as a scaffold.J Surg Res. 2001; 99: 352-358
- A biodegradable membrane from porcine intestinal submucosa to reinforce the gastrojejunostomy in laparoscopic Roux-en-Y gastric bypass: preliminary report.Obes Surg. 2001; 11: 469-473
- Small intestinal submucosa as a bioscaffold for biliary tract regeneration.Surgery. 2002; 132: 480-486
- Porcine small intestine submucosa as a treatment for enterocutaneous fistulas.J Am Coll Surg. 2002; 194: 541-543
- Evaluation of porcine-derived small intestine submucosa as a biodegradable graft for gastrointestinal healing.J Gastrointest Surg. 2003; 7: 96-101
- The use of small intestine submucosa in the repair of paraesophageal hernias: initial observations of a new technique.Am J Surg. 2003; 186: 4-8
- Clinical application of porcine small intestinal submucosa in the management of infected or potentially contaminated abdominal defects.J Gastrointest Surg. 2004; 8: 109-112
- Impaired expression of nitric oxide synthase in the gastric myenteric plexus of spontaneously diabetic rats.Gastroenterology. 1997; 113: 1535-1544
- Intracisternal TRH analog induces Fos expression in gastric myenteric neurons and glia in conscious rats.Am J Physiol Gastrointest Liver Physiol. 2001; 280: G979-G991
- S-100 proteins in the human peripheral nervous system.Microsc Res Tech. 2003; 60: 633-638
- Protein S100 immunoreactivity in glial cells and neurons of the Japanese quail brain.J Chem Neuroanat. 2003; 25: 195-212
- Detrusor regeneration in the rat using porcine small intestinal submucosal grafts: functional innervation and receptor expression.J Urol. 1996; 155: 374-378
- Characterization of small intestinal submucosa regenerated canine detrusor: assessment of reinnervation, in vitro compliance and contractility.J Urol. 1996; 156: 599-607
- Small intestinal submucosa as a vascular graft: a review.J Invest Surg. 1993; 6: 297-310
- Biocompatibility of small-intestinal submucosa in urinary tract as augmentation cystoplasty graft and injectable suspension.J Endourol. 1994; 8: 125-130
- The use of xenogeneic small intestinal submucosa as a biomaterial for Achilles tendon repair in a dog model.J Biomed Mater Res. 1995; 29: 977-985
- Small intestinal submucosa: utilization for repair of rodent abdominal wall defects.Ann Plast Surg. 1995; 35: 374-380
- Regenerative urinary bladder augmentation using small intestinal submucosa: urodynamic and histopathologic assessment in long-term canine bladder augmentations.J Urol. 1996; 155: 2098-2104
- Identification of extractable growth factors from small intestinal submucosa.J Cell Biochem. 1997; 67: 478-491
- Small intestinal submucosa as a large diameter vascular graft in the dog.J Surg Res. 1989; 47: 74-80
- Prevention of reperfusion injury in surgically induced gastric dilatation-volvulus in dogs.Am J Vet Res. 1990; 51: 294-299
- Histologic findings after in vivo placement of small intestine submucosal vascular grafts and saphenous vein grafts in the carotid artery in dogs.Am J Pathol. 1992; 140: 317-324
- Experimental assessment of small intestinal submucosa as a bladder wall substitute.Urology. 1995; 46: 396-400
- Regenerative bladder augmentation: a review of the initial preclinical studies with porcine small intestinal submucosa.Adv Exp Med Biol. 1995; 385: 229-235
- Laparoscopic ureteral reconstruction with small intestinal submucosa.J Endourol. 2001; 15: 217-220
- Ureteral segment replacement using a circumferential small-intestinal submucosa xenogenic graft.J Invest Surg. 2001; 14: 259-265
- Multilayered small intestinal submucosa is inferior to autologous bowel for laparoscopic bladder augmentation.J Urol. 2002; 168: 2253-2257
- Ureteral segmental replacement using multilayer porcine small-intestinal submucosa.J Endourol. 2002; 16: 27-31
- Small intestinal submucosa: a tissue-derived extracellular matrix that promotes tissue-specific growth and differentiation of cells in vitro.Tissue Eng. 1998; 4: 157-173
Ueno T, Oga A, Takahashi T, Pappas TN. Small intestinal submucosa (SIS) in the repair of a cecal wound in unprepared bowel in rats. J Gastrointest Surg. In press.
- Functional analysis of the tissue-engineered stomach wall.Artif Organs. 2002; 26: 868-872
Article info
Publication history
Accepted:
April 20,
2007
Footnotes
Supported by the Cook Company (makers of SIS).
Identification
Copyright
© 2007 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
