Background
The role of C-reactive protein (CRP), natural immunoglobulin M (IgM), and natural
IgM against phosphorylcholine (anti-Pc IgM) was investigated in relation with complement
activation in a rat model of intestinal ischemia and reperfusion (II/R). The effect
of C1-esterase inhibitor (C1-Inh) on this complement activation along with other inflammatory
mediators was also studied.
Methods
Rats were subjected to 1 h of superior mesenteric artery occlusion and 3 h of reperfusion.
Intravenous administration of vehicle (human albumin) or C1-Inh (200 U/kg) was performed
before (n = 8) or after ischemia (n = 8). II/R increased levels of C4b/c, CRP, IgM,
anti-Pc IgM, and myeloperoxidase activity in the intestinal homogenates and induced
vascular leakage.
Results
A good correlation was observed in the intestinal homogenates between C4b/c and CRP
levels. Clear depositions of C3, CRP, and IgM in intestinal tissue were demonstrated
after II/R, and a strong correlation of both CRP and IgM with complement was observed.
C1-Inh administered before ischemia reduced the complement activation response after
II/R, as reflected by decreased levels of C4b/c in conjunction with reduced anti-Pc
IgM in the intestinal homogenates. C1-Inh also decreased leakage of albumin when administered
before ischemia. C1-Inh after ischemia reduced C4b/c levels and myeloperoxidase activity
in the homogenates.
Conclusions
CRP and IgM depositions correlated well with local complement activation, which suggests
a role of these molecules in complement activation. Furthermore, C1-Inh inhibited potentially II/R injury either administered before or after ischemia, by attenuating
complement activation induced by CRP and/or natural IgM antibodies.
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Article info
Publication history
Accepted:
May 11,
2007
Identification
Copyright
© 2007 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
