Background
Chemokines are proinflammatory proteins that participate in immune and inflammatory
responses through chemoattraction and leukocyte activation. The chemokine antagonist
Met-RANTES (Regulated upon Activation Normal T cell Expressed and Secreted) has been
shown to be efficacious in decreasing inflammation in several animal disease models.
The purpose of this study was to investigate the effects of administration of Met-RANTES
on intra-abdominal adhesion formation after abdominal operation.
Materials and Methods
A 42 and 4-month-old female Wistar-Albino rats were subjected to standardized lesions
by cauterization of the cecum and uterine horn. They were divided randomly into 3
groups containing 14 rats each: group 1 (control), operative procedure without further
treatment; group 2 (Seprafilm), operative procedure with an antiadhesive membrane;
2 × 1 cm of Seprafilm was interposed beneath the peritoneal incision, and group 3
(Met-RANTES), operative procedure with the chemokine antagonist Met-RANTES. The extent
and severity of adhesions at the operative site were evaluated. Light microscopic
examination was performed to determine semiquantitative scores of VEGF expression.
Results
Rats in the control group formed extensive adhesions. In comparison with the control
group, the adhesion scores were significantly lower in the 2 other groups. The immunohistochemical
grading scores of vascular endothelial growth factor correlated closely with the total
adhesion scores and were less in groups 2 and 3 (P < .005).
Conclusions
Selective chemokine suppression with Met-RANTES seems to decrease rates, extent, and
severity of postoperative intraperitoneal adhesions.
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Article info
Publication history
Published online: July 11, 2008
Accepted:
April 16,
2008
Footnotes
Each author certifies that he or she has no commercial associations that may pose a conflict of interest in connection with submitted article, and that there is not any funding from outside agencies.
Identification
Copyright
© 2008 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
