Background
During liver ischemia, the decrease in mitochondrial energy causes cellular damage
that is aggravated after reperfusion. This injury can trigger a systemic inflammatory
syndrome, also producing remote organ damage. Several substances have been employed
to decrease this inflammatory response during liver transplantation, liver resections,
and hypovolemic shock. The aim of this study was to evaluate the effects of hypertonic
saline solution and the best timing of administration to prevent organ injury during
experimental liver ischemia/reperfusion.
Methods
Rats underwent 1 hr of warm liver ischemia followed by reperfusion. Eighty-four rats
were allocated into 6 groups: sham group, control of ischemia group (C), pre-ischemia
treated NaCl 0.9% (ISS) and NaCl 7.5% (HTS) groups, pre-reperfusion ISS, and HTS groups.
Blood and tissue samples were collected 4 hr after reperfusion.
Results
HTS showed beneficial effects in prevention of liver ischemia/reperfusion injury.
HTS groups developed increases in AST and ALT levels that were significantly less
than ISS groups; however, the HTS pre-reperfusion group showed levels significantly
less than the HTS pre-ischemia group. No differences in IL-6 and IL-10 levels were
observed. A significant decrease in mitochondrial dysfunction as well as hepatic edema
was observed in the HTS pre-reperfusion group. Pulmonary vascular permeability was
significantly less in the pre-reperfusion HTS group compared to the ISS group. No
differences in myeloperoxidase activity were observed. The liver histologic score
was significantly less in the pre-reperfusion HTS group compared to the pre-ischemia
HTS group.
Conclusion
HTS ameliorated local and systemic injuries in experimental liver ischemia/reperfusion.
Infusion of HTS in the pre-reperfusion period may be an important adjunct to accomplish
the best results.
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Article info
Publication history
Published online: December 11, 2009
Accepted:
October 5,
2009
Footnotes
This work was supported by grant 05/04226-7 from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).
Identification
Copyright
© 2010 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
