Background
Postconditioning may prove to be a suitable method to decrease ischemia-reperfusion
injury of intestine after mesenteric arterial occlusion. Toll-like-receptor-4 is involved
in the pathophysiology of organ damage after ischemia-reperfusion; therefore, the
aim of our study was to investigate the effect of postconditioning on the mucosal
expression of toll-like-receptor-4.
Methods
Male Wistar rats (n = 10/group) underwent 60 minutes of superior mesenteric artery occlusion followed
by 6 hours of reperfusion in 3 groups: sham-operated, ischemia-reperfusion, and a
postconditioned group. Postconditioning was performed by 6 alternating cycles of 10 seconds
of reperfusion/reocclusion. Blood and tissue samples were collected at the end of
reperfusion. Intestinal histopathologic changes and immunohistochemical expression
of mucosal caspase-3, antioxidant status, and protein levels of high-mobility group
box-1 and toll-like-receptor-4 were assessed. Immunofluorescent labeling and confocal
microscopic analysis of toll-like-receptor-4 were performed. Mucosal and serum levels
of interleukin-6 and tumor necrosis factor-α protein were measured.
Results
Histologic alterations in the postconditioned group were associated with decreased
caspase-3 positivity, less toll-like-receptor-4 mRNA, and less protein expression
of high-mobility group box-1 and toll-like-receptor-4 in the intestinal villi compared
with the ischemia-reperfusion group. Furthermore, a significantly improved antioxidant
state of the intestinal mucosa and less mucosal and serum protein levels of interleukin-6
and tumor necrosis factor-α were detected in the postconditioned group.
Conclusion
Small intestinal ischemia-reperfusion injury in male Wistar rats caused by the occlusion
of the superior mesenteric artery was ameliorated by the use of postconditioning,
showing a more favorable inflammatory response, which may be attributed to the decreased
mucosal expression of toll-like-receptor-4.
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Article info
Publication history
Published online: November 11, 2016
Accepted:
September 22,
2016
Identification
Copyright
© 2016 Elsevier Inc. All rights reserved.
