Abstract
Background
Previous data has shown that severe traumatic injury is associated with bone marrow
dysfunction, which manifests as persistent injury-associated anemia. This study sought
to identify whether the expression of erythropoiesis-related microRNAs were altered
in the bone marrow of trauma patients to determine if these microRNAs play a role
in persistent injury-associated anemia.
Methods
Bone marrow was collected from severely injured trauma patients who underwent fracture
fixation as well as patients who underwent elective hip replacement. There were 27
trauma patients and 10 controls analyzed. Total RNA and microRNA were isolated from
CD34-positive cells using the RNeasy Plus Mini kit, and genome-wide microRNA expression
patterns were assayed. Genes with significant expression differences were found using
BRB-ArrayTools with a significance of P < .01.
Results
There were marked differences in expression of 108 microRNAs in the trauma group when
compared with hip replacement patients. Four of these microRNAs play a role in regulating
erythropoiesis: microRNA-150, microRNA-223, microRNA15a, and microRNA-24. These microRNAs
were all upregulated significantly, with trauma/hip replacement fold changes of 1.7,
1.8, 1.2, and 1.2 respectively, and all act to suppress or regulate erythropoiesis.
Conclusion
Assessment of the bone marrow microRNA profile in trauma patients compared to those
undergoing elective hip replacement revealed the differential expression of microRNA-150,
microRNA-223, microRNA-15a, and microRNA-24. These microRNAs all play a role in decreased
erythroid progenitor cell growth and provide important insight to the erythropoietic
dysfunction seen after trauma.
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Article info
Publication history
Published online: January 04, 2021
Accepted:
November 16,
2020
Identification
Copyright
© 2020 Elsevier Inc. All rights reserved.
